Ecological Archives E089-078-A1

Timothy A. Nelson, Karalon Haberlin, Amorah V. Nelson, Heather Ribarich, Ruth Hotchkiss, Kathryn L. Van Alstyne, Lee Buckingham, Dejah J. Simunds, and Kerri Fredrickson. 2008. Ecological and physiological controls of species composition in green macroalgal blooms. Ecology 89:1287–1298.

Appendix A. Detailed site descriptions and expanded methods.

Table A1. Site locations.

Table A2. Sources of material for each experiment.

Table A3. Grazing experiment arena details.

TABLE A1. Site locations.

Area

Site

Latitude

Longitude

Blakely Island

     
 

West Beach

48°33.7’N

122°49.8’W

 

Armitage Bay

48°32.2’N

122°48.0’W

 

Driftwood Beach

48°35.3'N

122°48.5'W

 

Pinnacle Rock

48°32.6’N

122°47.1’W

 

Seal Pup Rock

48°35.2’N

122°47.9’W

       

San Juan Island

     
 

Friday Harbor Laboratories

48°32.7’N

123°00.6’W

 

Cattle Point

48°27.3’N

122°57.7’W

 

Brown Island

48°32.1’N

123°0.0’W

       

Shaw Island

     
 

Park’s Bay

48°33.5'N

122°58.8'W

 

Turn Point

48°33.4'N

122°59.1'W

       

Seattle

     
 

Lowman Beach

47°32.4’N

122°23.8’W

 

TABLE A2.  Sources of material for each experiment.

Experiment

Ulvaria
collection site

Ulva
collection site

Animal
collecting site

Field Microcosm Experiments

Armitage Bay

Armitage Bay

N/A

Desiccation

Seal Pup Rock

Seal Pup Rock

N/A

Light Acclimation:  Photosynthesis

Armitage Bay

Armitage Bay

N/A

Light Acclimation: Respiration

Cattle Point

Friday Harbor Labs

N/A

Salinity

Lowman Beach

Lowman Beach

N/A

N additions and growth, tissue C and N

Lowman Beach

Lowman Beach

N/A

N additions and growth rate, pigments and proteins

Parks Bay

Parks Bay

N/A

Grazing Choice: Littorina litkana

Armitage Bay

Armitage Bay

Driftwood Beach

Grazing Choice: Lacuna variegata

Brown Island

Friday Harbor

Brown Island

Grazing Choice: Lacuna vincta

Cattle Point

Friday Harbor

Turn Point

Grazing Choice: Idotea wosnesenskii and
Strongylocentrotus droebachiensis

Cattle Point

Friday Harbor

Cattle Point

No-choice Grazing and Acclimated Grazing

Cattle Point

Friday Harbor

Cattle Point

Beach/Tidepool Grazing: Cattle Point

Cattle Point

Friday Harbor

N/A

Beach/Tidepool Grazing: Pinnacle Rock

Armitage Bay

Armitage Bay

N/A

 

TABLE A3. Grazing experiment arena details.

Experiment

Grazer

Arena description

Grazers per arena

No. arenas

Incubation period

Grazing Choice

Littorina sitkana

Aerated 600 mL tissue culture flasks in a temperature-controlled chamber

25

23

4 days

Grazing Choice

Lacuna vincta

600 mL tissue culture flasks in a sea table, with seawater flowing through the flask.

30

15

7 days

Grazing Choice

Lacuna variegata

600 mL tissue culture flasks in a sea table with seawater flowing through the flask.

30

15

7 days

Grazing Choice

Idotea wosnesenskii

30 cm long sections of 10 cm diameter polyvinylchloride (PVC) pipe. The bottom of each pipe was covered with nytex mesh. Two 8 cm × 10 cm holes were cut in the sides of the pipe and coveredwith screen to allow for water exchange.

1

15

6 days

Grazing Choice

Strongylocentrotus droebachiensis (<5 cm test diameter)

15 cm × 15 cm × 20 cm (l × w × h) mesh cages (1 cm mesh size) placed in a sea table.

1-2

15

4 days

No-choice

Lacuna vincta

600 mL tissue culture flasks in a sea table with seawater flowing through the flask.

30

6 for Ulva, 5 for Ulvaria

7 days

Pre-conditioned

Lacuna vincta

600 mL tissue culture flasks in a sea table with seawater flowing through the flask.

30

6 for Ulva, 5 for Ulvaria

7 days


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