Ecological Archives C006-092-A3
Rogelio Cruz-Reyes, Germán Ávila-Sakar, Gumersindo Sánchez-Montoya, and Mauricio Quesada. 2015. Experimental assessment of gene flow between transgenic squash and a wild relative in the center of origin of cucurbits. Ecosphere 6:248. http://dx.doi.org/10.1890/es15-00304.1
Appendix C. Number of transgenic plants per family and comparisons of performance of hybrids, between plants VRT and non-VRT.
Table C1. Number of hybrids F1 transgenic (TRV) and non transgenic (non-TRV) per family.
|
Pollination treatment |
|
||||||
|
Saturation |
15 : 1 |
7:1 |
3:1 |
||||
Families |
TRV |
non-TRV |
TRV |
non-TRV |
TRV |
non-TRV |
TRV |
non-TRV |
1 |
15 |
18 |
12 |
15 |
4 |
3 |
3 |
10 |
2 |
17 |
23 |
6 |
3 |
3 |
8 |
3 |
40 |
3 |
56 |
44 |
4 |
4 |
6 |
27 |
7 |
2 |
4 |
18 |
20 |
34 |
43 |
4 |
17 |
10 |
30 |
5 |
8 |
4 |
14 |
11 |
3 |
19 |
3 |
13 |
6 |
35 |
36 |
7 |
6 |
1 |
7 |
2 |
14 |
7 |
17 |
13 |
24 |
19 |
11 |
11 |
4 |
21 |
8 |
4 |
6 |
10 |
20 |
5 |
10 |
4 |
27 |
9 |
11 |
9 |
9 |
4 |
4 |
25 |
1 |
10 |
10 |
12 |
11 |
3 |
4 |
1 |
6 |
5 |
23 |
11 |
9 |
4 |
10 |
7 |
2 |
15 |
1 |
4 |
12 |
4 |
5 |
5 |
13 |
2 |
15 |
3 |
4 |
13 |
6 |
8 |
3 |
8 |
|
|
5 |
28 |
14 |
6 |
9 |
14 |
32 |
|
|
4 |
19 |
15 |
20 |
22 |
6 |
22 |
|
|
2 |
15 |
16 |
16 |
25 |
7 |
1 |
|
|
3 |
14 |
17 |
33 |
38 |
|
|
|
|
15 |
60 |
18 |
13 |
20 |
|
|
|
|
|
|
19 |
9 |
13 |
|
|
|
|
|
|
20 |
50 |
51 |
|
|
|
|
|
|
21 |
30 |
41 |
|
|
|
|
|
|
22 |
4 |
7 |
|
|
|
|
|
|
23 |
9 |
10 |
|
|
|
|
|
|
24 |
72 |
86 |
|
|
|
|
|
|
25 |
8 |
10 |
|
|
|
|
|
|
26 |
9 |
5 |
|
|
|
|
|
|
27 |
12 |
15 |
|
|
|
|
|
|
28 |
19 |
22 |
|
|
|
|
|
|
29 |
6 |
11 |
|
|
|
|
|
|
30 |
4 |
7 |
|
|
|
|
|
|
31 |
9 |
5 |
|
|
|
|
|
|
32 |
10 |
12 |
|
|
|
|
|
|
33 |
55 |
72 |
|
|
|
|
|
|
34 |
14 |
24 |
|
|
|
|
|
|
35 |
3 |
14 |
|
|
|
|
|
|
36 |
9 |
24 |
|
|
|
|
|
|
37 |
13 |
37 |
|
|
|
|
|
|
38 |
21 |
37 |
|
|
|
|
|
|
39 |
5 |
24 |
|
|
|
|
|
|
40 |
3 |
11 |
|
|
|
|
|
|
Table C2. Number of hybrids F2 transgenic (TRV) and non transgenic (non-TRV) per families.
|
Pollination treatment |
|||
|
F1 non-TRV × F1 TRV |
F1 TRV × F1 TRV |
||
Families |
TRV |
non-TRV |
TRV |
non-TRV |
1 |
12 |
13 |
18 |
15 |
2 |
7 |
10 |
7 |
16 |
3 |
42 |
3 |
46 |
24 |
4 |
9 |
20 |
26 |
6 |
5 |
7 |
6 |
16 |
17 |
6 |
5 |
8 |
32 |
10 |
7 |
18 |
52 |
31 |
19 |
8 |
14 |
39 |
52 |
13 |
9 |
13 |
27 |
28 |
15 |
10 |
9 |
17 |
23 |
8 |
11 |
16 |
11 |
21 |
1 |
12 |
28 |
24 |
10 |
1 |
13 |
26 |
30 |
9 |
1 |
14 |
43 |
47 |
17 |
22 |
15 |
20 |
19 |
12 |
9 |
16 |
17 |
13 |
47 |
17 |
17 |
5 |
5 |
17 |
12 |
18 |
12 |
12 |
49 |
71 |
19 |
42 |
56 |
28 |
16 |
20 |
28 |
33 |
|
|
21 |
29 |
26 |
|
|
22 |
10 |
3 |
|
|
23 |
28 |
49 |
|
|
24 |
50 |
38 |
|
|
25 |
16 |
30 |
|
|
26 |
31 |
19 |
|
|
27 |
13 |
27 |
|
|
Table C3. Number of transgenic (TRV) backcross plants and non transgenic (non-TRV) per families.
|
Pollination treatment |
|||
|
F1 VRT × W |
W × F1 VRT |
||
Families |
TRV |
non-TRV |
TRV |
non-TRV |
1 |
4 |
7 |
14 |
6 |
2 |
14 |
17 |
5 |
4 |
3 |
22 |
23 |
16 |
18 |
4 |
4 |
7 |
|
|
Table C4. Multivariate analyses of variance (MANOVA) for the effects of the type of cross (see Table A1) and transgene (presence / absence) on the performance of F1 and F2 hybrid progeny.
Source |
df |
Wilk’s λ |
F |
P |
F1 generation |
|
|
|
|
Type of cross |
15, 97 |
0.745 |
0.727 |
0.751 |
Transgene |
5, 35 |
0.835 |
1.382 |
0.255 |
F2 generation |
|
|
|
|
Type of cross |
4, 17 |
0.611 |
2.69 |
0.066 |
Transgene |
4, 17 |
0.713 |
1.71 |
0.194 |
Table C4 (continued)
F1 Eigen Analysis for Type of cross |
|||||
Eigenvalue |
0.2041 |
0.07292 |
0.03884 |
0.00000 |
0.00000 |
Proportion |
0.6461 |
0.23089 |
0.12300 |
0.00000 |
0.00000 |
Cumulative |
0.6461 |
0.87700 |
1.00000 |
1.00000 |
1.00000 |
Eigenvector |
1 |
2 |
3 |
4 |
5 |
Days to emergence |
-0.3409 |
-3.200 |
-1.345 |
0.179 |
-0.188 |
Pistillate flowers |
0.1435 |
-0.192 |
0.021 |
-0.091 |
-0.099 |
Staminate flowers |
-0.4458 |
0.518 |
-0.193 |
0.183 |
-0.491 |
Seeds per fruit |
-0.1889 |
-0.072 |
-0.012 |
-0.245 |
0.043 |
Seed mass |
1.0807 |
0.688 |
-2.467 |
0.000 |
0.471 |
Eigen Analysis for Transgene |
|||||
Eigenvalue |
0.1974 |
0.00000 |
0.00000 |
0.00000 |
0.00000 |
Proportion |
1.0000 |
0.00000 |
0.00000 |
0.00000 |
0.00000 |
Cumulative |
1.0000 |
1.00000 |
1.00000 |
1.00000 |
1.00000 |
Eigenvector |
1 |
2 |
3 |
4 |
5 |
Days to emergence |
-1.380 |
-0.394 |
2.948 |
0.572 |
1.076 |
Pistillate flowers |
-0.080 |
-0.068 |
0.165 |
-0.193 |
0.025 |
Staminate flowers |
-0.406 |
-0.145 |
-0.685 |
0.344 |
0.079 |
Seeds per fruit |
-0.067 |
-0.111 |
0.112 |
0.107 |
-0.249 |
Seed mass |
-1.205 |
2.549 |
0.000 |
0.000 |
0.000 |
F2 Eigen Analysis for Type of cross |
||||
Eigenvalue |
0.6347 |
0.00000 |
0.00000 |
0.00000 |
Proportion |
1.0000 |
0.00000 |
0.00000 |
0.00000 |
Cumulative |
1.0000 |
1.00000 |
1.00000 |
1.00000 |
Eigenvector |
1 |
2 |
3 |
4 |
Days to emergence |
1.840 |
2.860 |
2.697 |
-0.062 |
Foliar area at d 14 |
-0.075 |
-0.577 |
0.817 |
-0.728 |
Pistillate flowers |
-0.621 |
-0.029 |
0.034 |
0.143 |
Staminate flowers |
-0.677 |
0.000 |
0.000 |
-1.115 |
Eigen Analysis for Transgene |
||||
Eigenvalue |
0.4023 |
0.00000 |
0.00000 |
0.00000 |
Proportion |
1.0000 |
0.00000 |
0.00000 |
0.00000 |
Cumulative |
1.0000 |
1.00000 |
1.00000 |
1.00000 |
Eigenvector |
1 |
2 |
3 |
4 |
Days to emergence |
3.339 |
0.220 |
2.517 |
1.145 |
Foliar area at d 14 |
-0.016 |
-0.645 |
0.693 |
-0.801 |
Pistillate flowers |
0.491 |
-0.330 |
-0.217 |
0.105 |
Staminate flowers |
-0.758 |
0.000 |
0.000 |
-1.062 |
Table C5. Two-way univariate analyses of variance for the effects of the type of backcross (see Table A1), transgene (presence / absence), and their interaction on the performance of backcross progeny.
Source |
Days to germination |
Leaf area |
Number of pistillate flowers |
Number of staminate flowers |
||||||||
|
df |
F |
P |
d.f |
F |
P |
df |
F |
P |
df |
F |
P |
Type of backcross (B) |
1 |
1.50 |
0.222 |
1 |
0.73 |
0.401 |
1 |
0.005 |
0.965 |
1 |
0.05 |
0.823 |
Transgene (T) |
1 |
0.09 |
0.758 |
1 |
2.80 |
0.108 |
1 |
0.02 |
0.892 |
1 |
0.59 |
0.446 |
B × T |
1 |
0.13 |
0.721 |
1 |
0.23 |
0.639 |
1 |
0.06 |
0.804 |
1 |
3.28 |
0.078 |
Error |
186 |
|
|
23 |
|
|
23 |
|
|
38 |
|
|
Total |
189 |
|
|
26 |
|
|
26 |
|
|
41 |
|
|
Note: Variables analyzed were days to germination, leaf area (cm²)14 days after emergence, number of pistillate, and staminate flowers produced.