Ecological Archives C006-032-A4

Cheng-Yuan Xu, Shaoqing Tang, Mohammad Fatemi, Caroline L. Gross, Mic H. Julien, Caitlin Curtis, and Rieks D. van Klinken . 2015. Population structure and genetic diversity of invasive Phyla canescens: implications for the evolutionary potential. Ecosphere 6:162. http://dx.doi.org/10.1890/es14-00374.1

Appendix D. A table showing power analysis of microsatellite data with POWSIM (Ryman, 2011, Userís manual of POWSIM, a computer program for assessing statistical power when testing for genetic homogeneity, Version 4.1).

Table D1. Power analysis of microsatellite data with POWSIM (Ryman, 2011, User’s manual of POWSIM, a computer program for assessing statistical power when testing for genetic homogeneity, Version 4.1). The test was conducted for all 32 populations used in other analyses (including 5 pooled populations in the native range) and 47 non-pooled native populations (with 1–30 individuals collected per site), respectively. Five levels of population differentiation (Fst = 0.001, 0.0025, 0.005, 0.01, 0.025) were pre-defined with effective population sizes Ne = 1000 and Ne = 10,000 and the simulation was replicated 1000 times. Fisher’s exact P values are shown. The results suggest that our data can effectively distinguish genetic differentiation corresponding to Fst ≥ 0.01, which provides sufficient power to address the broad-scale spatial genetic structure of P. canescens.

Populations

Effective population size (Ne)

Fst

0.001

0.0025

0.005

0.01

0.025

All (n=32)

1000

0.31

0.82

1.00

1.00

1.00

10000

0.27

0.81

1.00

1.00

1.00

Native (before pooling, n=47)

1000

0.20

0.45

0.87

1.00

1.00

10000

0.17

0.43

0.88

1.00

1.00


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