Ecological Archives E096-214-A1

Meredith L. Mendola, Sara G. Baer, Loretta C. Johnson, and Brian R. Maricle. 2015. The role of ecotypic variation and the environment on biomass and nitrogen in a dominant prairie grass. Ecology 96:2433–2445. http://dx.doi.org/10.1890/14-1492.1

Appendix A. Statistical results from the ‘sympatric vs. allopatric’ tests for local adaption.

Table A1. Least squares mean plant response by site and ecotype (populations averaged within ecotypes) and analysis of variance (ANOVA) results from tests of similar mean distribution of ecotype response in common gardens sympatric or allopatric from their region of origin. Biomass (aboveground and root) and belowground net primary productivity (BNPP) were log-transformed prior to performing the ‘sympatric v. allopatric’ (SA) contrast. Total degrees of freedom for all models was 8 (model = 5, error = 3, site = 2, ecotype = 2, and SA contrast = 1).

 

Least Squares Means of Each Ecotype at Each Site

Site

Ecotype

Contrast (SA)

Above-ground Biomass
(g plant-1)

Root Biomass
(g m-2)

BNPP
(g m-2 gs-1)

N Storage in Roots
(g m-2)

 

 

 

 

 

 

 

S1cks

CKS

Sympatric

   439.5

   38.7

119.1

0.584

S1cks

EKS

Allopatric

   350.3

   44.8

140.8

0.647

S1cks

SIL

Allopatric

   437.7

110.8

235.7

1.916

S2eks

CKS

Allopatric

   471.3

   32.6

103.1

0.304

S2eks

EKS

Sympatric

   648.0

   81.1

176.0

0.924

S2eks

SIL

Allopatric

1086.8

134.0

449.2

1.653

S3sil

CKS

Allopatric

   476.7

   21.3

   33.3

0.211

S3sil

EKS

Allopatric

   685.1

   39.2

   56.9

0.535

S3sil

SIL

Sympatric

1420.5

216.8

341.5

2.515

 

Analysis of Variance Results

 

Sums of Squares

Mean Square Error

F value

P value

 

 

 

 

 

 

Aboveground Biomass

Model

1.573

0.315

      6.59

0.076

 

Error

0.143

0.048

 

 

 

Site

0.708

0.354

      7.42

0.069

 

Ecotype

0.674

0.337

      7.05

0.073

 

SA

0.191

0.191

      4.00

0.140

 

 

Root Biomass

 

Model

4.608

0.922

   53.35

0.004

  

 

Error

0.052

0.017

 

 

 

site

0.092

0.046

     2.66

0.217

 

ecotype

3.934

1.967

113.86

0.002

 

SA

0.582

0.582

  33.70

0.010

 

 

BNPP

 

Model

5.512

1.102

    7.74

0.061

  

 

Error

0.427

0.142

 

 

 

site

0.850

0.425

     2.98

0.194

 

ecotype

4.020

2.010

   14.11

0.030

 

SA

0.643

0.643

     4.52

0.124

 

 

N Storage in Roots

Model

5.089

1.018

   33.59

0.008

 

Error

0.091

0.030

 

 

 

site

0.025

0.013

      0.42

0.692

 

ecotype

4.634

2.317

   76.47

0.003

 

SA

0.430

0.430

   14.18

0.033


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