Ecological Archives E096-082-A5

Cyrus A. Mallon, Franck Poly, Xavier Le Roux, Irene Marring, Jan Dirk Van Elsas, and Joana Falcão Salles. 2015. Resource pulses can alleviate the biodiversity–invasion relationship in soil microbial communities. Ecology 96:915–926.

Appendix E. Quantification and calculation of community resource use and remaining niche breadth of the invader: Biolog’s high cell density phenotypic microarray protocol.

Specifically, in accordance with Biolog's high cell density phenotypic microarray protocol, 2 mL of a 1000× soil dilution, 8 mL of biolog's IF-0a inoculation fluid, 0.12 mL of biolog Dye D, and 1.88 mL of sterile demineralized water were used to inoculate one biolog GEN III plate. 100 µL of this mix was inoculated into each well of the GEN III plate. Two replicate plates were used for each microcosm. Carbon source utilization was measured every 15 minutes for 48 hours in Biolog's Omnilog machine, similarly to the protocol used for bacterial strains.

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