Ecological Archives E089-078-A1

Ecological Archives E089-078-A1

Timothy A. Nelson, Karalon Haberlin, Amorah V. Nelson, Heather Ribarich, Ruth Hotchkiss, Kathryn L. Van Alstyne, Lee Buckingham, Dejah J. Simunds, and Kerri Fredrickson. 2008. Ecological and physiological controls of species composition in green macroalgal blooms. Ecology 89:1287–1298.

Appendix A. Detailed site descriptions and expanded methods.

Table A1. Site locations.

Table A2. Sources of material for each experiment.

Table A3. Grazing experiment arena details.

TABLE A1. Site locations.

Area	Site	Latitude	Longitude
Blakely Island
	West Beach	48°33.7’N	122°49.8’W
	Armitage Bay	48°32.2’N	122°48.0’W
	Driftwood Beach	48°35.3'N	122°48.5'W
	Pinnacle Rock	48°32.6’N	122°47.1’W
	Seal Pup Rock	48°35.2’N	122°47.9’W

San Juan Island
	Friday Harbor Laboratories	48°32.7’N	123°00.6’W
	Cattle Point	48°27.3’N	122°57.7’W
	Brown Island	48°32.1’N	123°0.0’W

Shaw Island
	Park’s Bay	48°33.5'N	122°58.8'W
	Turn Point	48°33.4'N	122°59.1'W

Seattle
	Lowman Beach	47°32.4’N	122°23.8’W

TABLE A2. Sources of material for each experiment.

Experiment	Ulvaria collection site	Ulva collection site	Animal collecting site
Field Microcosm Experiments	Armitage Bay	Armitage Bay	N/A
Desiccation	Seal Pup Rock	Seal Pup Rock	N/A
Light Acclimation: Photosynthesis	Armitage Bay	Armitage Bay	N/A
Light Acclimation: Respiration	Cattle Point	Friday Harbor Labs	N/A
Salinity	Lowman Beach	Lowman Beach	N/A
N additions and growth, tissue C and N	Lowman Beach	Lowman Beach	N/A
N additions and growth rate, pigments and proteins	Parks Bay	Parks Bay	N/A
Grazing Choice: Littorina litkana	Armitage Bay	Armitage Bay	Driftwood Beach
Grazing Choice: Lacuna variegata	Brown Island	Friday Harbor	Brown Island
Grazing Choice: Lacuna vincta	Cattle Point	Friday Harbor	Turn Point
Grazing Choice: Idotea wosnesenskii and Strongylocentrotus droebachiensis	Cattle Point	Friday Harbor	Cattle Point
No-choice Grazing and Acclimated Grazing	Cattle Point	Friday Harbor	Cattle Point
Beach/Tidepool Grazing: Cattle Point	Cattle Point	Friday Harbor	N/A
Beach/Tidepool Grazing: Pinnacle Rock	Armitage Bay	Armitage Bay	N/A

TABLE A3. Grazing experiment arena details.

Experiment	Grazer	Arena description	Grazers per arena	No. arenas	Incubation period
Grazing Choice	Littorina sitkana	Aerated 600 mL tissue culture flasks in a temperature-controlled chamber	25	23	4 days
Grazing Choice	Lacuna vincta	600 mL tissue culture flasks in a sea table, with seawater flowing through the flask.	30	15	7 days
Grazing Choice	Lacuna variegata	600 mL tissue culture flasks in a sea table with seawater flowing through the flask.	30	15	7 days
Grazing Choice	Idotea wosnesenskii	30 cm long sections of 10 cm diameter polyvinylchloride (PVC) pipe. The bottom of each pipe was covered with nytex mesh. Two 8 cm × 10 cm holes were cut in the sides of the pipe and coveredwith screen to allow for water exchange.	1	15	6 days
Grazing Choice	Strongylocentrotus droebachiensis (<5 cm test diameter)	15 cm × 15 cm × 20 cm (l × w × h) mesh cages (1 cm mesh size) placed in a sea table.	1-2	15	4 days
No-choice	Lacuna vincta	600 mL tissue culture flasks in a sea table with seawater flowing through the flask.	30	6 for Ulva, 5 for Ulvaria	7 days
Pre-conditioned	Lacuna vincta	600 mL tissue culture flasks in a sea table with seawater flowing through the flask.	30	6 for Ulva, 5 for Ulvaria	7 days

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