Ecological Archives A025-074-A1
Yuting Liang, Xu Zhang, Jizhong Zhou, and Guanghe Li. 2015. Long-term oil contamination increases deterministic assembly processes in soil microbes. Ecological Applications 25:1235–1243. http://dx.doi.org/10.1890/14-1672.1
Appendix A. Tables and figures showing dissimilarity among all the five sites and between pairwise sites by NPMANOVA (Table A1, A2), and decrease of microbial functional genes richness (Fig. A1) and diversity (Fig. A2–A6) along with long-term oil contamination.
Table A1. Dissimilarity test among all the five sites by NPMANOVA.
|
r value |
P |
Uncontaminated soils |
2.880 |
< 0.0001 |
Contaminated soils |
1.601 |
0.0002 |
Table A2. Dissimilarity test between pairwise sites by NPMANOVA.
Uncontaminated soils |
|
Contaminated soils |
|||||||||
r value\P |
BS |
DQ |
JH |
SL |
TLM |
|
BS |
DQ |
JH |
SL |
TLM |
BS |
|
0.0002 |
0.0007 |
0.0001 |
0.0002 |
|
|
0.1427 |
0.0106 |
0.1611 |
0.0305 |
DQ |
2.619 |
|
0.0002 |
0.0002 |
0.0003 |
|
1.191 |
|
0.0176 |
0.0726 |
0.0004 |
JH |
2.980 |
2.553 |
|
0.0019 |
0.0001 |
|
1.817 |
1.617 |
|
0.0266 |
0.0001 |
SL |
2.658 |
2.959 |
2.260 |
|
0.0098 |
|
1.223 |
1.418 |
1.723 |
|
0.1560 |
TLM |
4.235 |
3.938 |
2.979 |
2.051 |
|
|
1.612 |
1.937 |
2.320 |
1.228 |
|
Fig. A1. Microbial functional genes (richness) decrease significantly with long-term oil contamination.
Fig. A2. Decrease in microbial diversity (Shannon index) of all functional genes with oil concentrations across the five sites.
Fig. A3. Decrease in microbial diversity (Shannon index) of functional genes in nitrogen cycling with oil concentrations across the five sites. nifH is active in nitrogen fixation, and nirS/K, nos in denitrification.
Fig. A4. Decrease in microbial diversity (Shannon index) of functional genes in carbon fixation with oil concentrations across the five sites. Rubisco (ribulose-l, 5-bisphosphate carboxylase/oxygenase) is expressed in the Calvin cycle, CODH (carbon monoxide dehydrogenase) in the reductive acetyl-CoA pathway, and pcc (propionyl-CoA/acetyl-CoA carboxylase) in the 3-hydroxypropionate/malyl-CoA cycle.
Fig. A5. Decrease in microbial diversity (Shannon index) of functional genes in carbon degradation (starch, cellulose, chitin and lignin) with oil concentrations across the five sites.
Fig. A6. Decrease in microbial diversity (Shannon index) of functional genes in phosphorus utilization with oil concentrations across the five sites. ppk and ppx encode polyphosphate kinase and exopolyphosphatase respectively.
