*Ecological Archives* E096-081-A2

Jörg G. Stephan, Johan A. Stenberg, and Christer Björkman. 2015. How far away is the next basket of eggs? Spatial memory and perceived cues shape aggregation patterns in a leaf beetle. *Ecology* 96:908–914. http://dx.doi.org/10.1890/14-1143.1

Appendix B. Decreasing distance with increasing number of clutches and believed oviposition pattern.

Fig. B1. Box plot of intra-plant distances between clutches depending on the number of clutches on a plant. Irrespective of plant genotype, the mean distance between clutches was dependent on the number of clutches on one shoot (Linear mixed model with plant as random effect: clutch number: Chi-square = 14.2, df = 1, *p* < 0.001; plant species: Chi-square = 1.1, df = 1, *p* = 0.29; clutch number × plant species: Chi-square = 0.7, df = 1, *p* = 0.38).

Fig. B2. Original distances between clutches from all plants. These distances were standardized by the number of clutches on a plant and further divided by the total plant leaf area of each plant (Mean, SD, Min, Max leaf area (mm²) of one leaf: *S. viminalis*: 2186, 450, 1047, 3469; *S. dasyclados*: 4880, 1591, 1518, 10260).

Fig. B3. Example calculation of believed oviposition patterns for each female during the experiment with four females (all spent the same amount of time on a plant, and fewer eggs implies a lower oviposition rate (OR)). The first females laid the same number of eggs, but subsequent females laid fewer. (a) The OR decreases with increasing number of eggs on a plant. (b) The OR decreases with number of conspecifics visiting a plant; the number of conspecifics increases faster on the *new host *then on the *mixed hosts* and, therefore, the OR decrease was greater in the *new hosts* treatment. (c) The cumulative OR decreases due to number of eggs and due to number of conspecifics. (d) The observed highest egg numbers in the *mixed hosts* treatment must originate from a higher OR although the egg number on a plant was increasing, otherwise *mixed hosts* would have received as many eggs as the *same host* treatment. Therefore, females in this treatment must have ranked the preferred intermediate conspecific density higher than the higher number of eggs on a plant.