Ecological Archives E089-021-A2

Ian Kaplan, Rayko Halitschke, André Kessler, Sandra Sardanelli, and Robert F. Denno. 2008. Constitutive and induced defenses to herbivory in above- and belowground plant tissues. Ecology 89:392–406.

Appendix B. MANOVA table for the effects of root herbivory (nematodes), shoot herbivory (generalist and specialist caterpillars), and their interaction on above- and belowground secondary chemistry in Nicotiana tabacum.

   

Meloidogyne incognita
(root-galling nematode)

 

Manduca sexta
(specialist caterpillar)

 

Trichoplusia ni
(generalist caterpillar)

Tissue

Compound

  df

     F

  P

 

df

   F

P

 

df

 F

P

Leaf

MANOVA

6, 71

20.74

<0.0001

 

6, 71

6.73

<0.0001

 

6, 71

32.11

<0.0001

 

Nicotine

1, 63

79.92

<0.0001

 

1, 63

5.98

0.0173

 

1, 63

20.05

<0.0001

 

Non-nicotine alkaloids

1, 63

24.31

<0.0001

 

1, 63

5.07

0.0279

 

1, 63

8.48

0.0050

 

Chlorogenic acid

1, 63

4.57

0.0365

 

1, 63

1.54

0.2192

 

1, 63

0.38

0.5373

 

Rutin

1, 63

11.83

0.0010

 

1, 63

10.10

0.0023

 

1, 63

8.70

0.0045

 

Phenolic-2

1, 63

7.20

0.0093

 

1, 63

0.37

0.5469

 

1, 63

1.570

0.2145

 

Diterpene glycosides

1, 63

6.22

0.0153

 

1, 63

20.46

<0.0001

 

1, 63

153.62

<0.0001

Root

MANOVA

6, 68

10.74

<0.0001

 

6, 68

1.22

0.3061

 

6, 68

1.21

0.3119

 

Nicotine

1, 60

20.10

<0.0001

 

1, 60

0.23

0.6301

 

1, 60

0.14

0.7082

 

Non-nicotine alkaloids

1, 60

14.80

0.0003

 

1, 60

0.01

0.9304

 

1, 60

0.07

0.7910

 

Chlorogenic acid

1, 60

0.00

0.9937

 

1, 60

0.28

0.6006

 

1, 60

0.84

0.3618

 

Rutin

1, 60

1.61

0.2099

 

1, 60

0.60

0.4400

 

1, 60

1.72

0.1945

 

Phenolic-2

1, 60

6.44

0.0138

 

1, 60

0.99

0.3230

 

1, 60

0.59

0.4441

 

Phenolic-1

1, 60

8.25

0.0056

 

1, 60

0.16

0.6950

 

1, 60

0.06

0.8144

Gall

MANOVA

6, 66

220.67

<0.0001

 

6, 66

0.42

0.8605

 

6, 66

1.48

0.1976

 

Nicotine

1, 58

79.14

<0.0001

 

1, 58

0.14

0.7072

 

1, 58

0.00

0.9893

 

Non-nicotine alkaloids

1, 58

196.64

<0.0001

 

1, 58

0.20

0.6526

 

1, 58

0.69

0.4110

 

Chlorogenic acid

1, 58

7.44

0.0084

 

1, 58

1.44

0.2343

 

1, 58

4.41

0.0401

 

Rutin

1, 58

1353.55

<0.0001

 

1, 58

0.00

0.9807

 

1, 58

0.04

0.8353

 

Phenolic-2

1, 58

650.91

<0.0001

 

1, 58

0.00

0.9999

 

1, 58

0.12

0.7291

 

Phenolic-1

1, 58

199.43

<0.0001

 

1, 58

1.37

0.2469

 

1, 58

2.82

0.0985

Table 1 (continued)

   

Manduca sexta
×
Meloidogyne incognita

 

Trichoplusia ni
×
Meloidogyne incognita

Tissue

Compound

  df

     F

  P

 

df

F

P

Leaf

MANOVA

6, 71

1.26

0.2877

 

6, 71

4.59

0.0005

 

Nicotine

1, 63

2.61

0.1114

 

1, 63

7.35

0.0087

 

Non-nicotine alkaloids

1, 63

0.94

0.3360

 

1, 63

1.55

0.2181

 

Chlorogenic acid

1, 63

0.66

0.4184

 

1, 63

20.23

<0.0001

 

Rutin

1, 63

0.84

0.3636

 

1, 63

15.37

0.0002

 

Phenolic-2

1, 63

0.46

0.4987

 

1, 63

0.61

0.4377

 

Diterpene glycosides

1, 63

2.33

0.1320

 

1, 63

0.01

0.9048

Root

MANOVA

6, 68

0.86

0.5281

 

6, 68

2.27

0.0466

 

Nicotine

1, 60

0.58

0.4493

 

1, 60

0.16

0.6908

 

Non-nicotine alkaloids

1, 60

0.03

0.8598

 

1, 60

1.10

0.2989

 

Chlorogenic acid

1, 60

0.20

0.6566

 

1, 60

0.00

0.9452

 

Rutin

1, 60

0.24

0.6251

 

1, 60

1.61

0.2089

 

Phenolic-2

1, 60

0.00

0.9951

 

1, 60

1.45

0.2340

 

Phenolic-1

1, 60

1.47

0.2294

 

1, 60

4.92

0.0303

Gall

MANOVA

6, 66

1.47

0.2029

 

6, 66

1.67

0.1432

 

Nicotine

1, 58

0.30

0.5843

 

1, 58

0.81

0.3725

 

Non-nicotine alkaloids

1, 58

0.66

0.4183

 

1, 58

0.01

0.9061

 

Chlorogenic acid

1, 58

1.62

0.2088

 

1, 58

2.43

0.1242

 

Rutin

1, 58

0.33

0.5669

 

1, 58

0.00

0.9541

 

Phenolic-2

1, 58

1.32

0.2547

 

1, 58

0.02

0.8826

 

Phenolic-1

1, 58

0.16

0.6887

 

1, 58

0.48

0.4896

The P values for significant effects (P < 0.05) are highlighted in bold font.



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