Appendix B. Summary of worker heterozygosity data for two allozymes (PGI and EST-1) sampled across populations of Pogonomyrmex barbatus and P. rugosus.
B and R represent environmental caste determination (ECD) lineages; J1/J2 and H1/H2 represent dependent lineages as determined by association of morphology, nuclear genotype and mitotype (see Table 1 and text). Hetero-workers is the proportion of heterozygous workers in each population for each allozyme. Three locations contained both GCD and ECD colonies (37, 41, 43), with the number of each colony type in parentheses. Chi-square and P values were assessed by selecting one random worker per colony, then comparing the number of heterozygotes to that expected at Hardy-Weinberg equilibrium (see text). See Table 1 for location information and sample size.
Location |
Lineage |
PGI Hetero- |
χ2 |
P |
EST-1 Hetero- |
χ2 |
P |
P. barbatus |
|||||||
1. AZ: Yavapai |
J1/J2 |
1.00 |
10.0 |
0.002 |
0.77 |
1.9 |
ns |
2. AZ: Yavapai |
J1/J2 |
1.00 |
15.0 |
0.0001 |
0.76 |
2.1 |
ns |
3. AZ: Pinal |
J1/J2 |
1.00 |
4.0 |
0.05 |
|||
4. AZ: Maricopa |
J1/J2 |
1.00 |
10.0 |
0.002 |
0.68 |
1.8 |
ns |
5. AZ: Pinal |
J1/J2 |
1.00 |
10.0 |
0.002 |
0.48 |
0.8 |
ns |
6. AZ: Santa Cruz |
J1/J2 |
1.00 |
11.0 |
0.0005 |
0.33 |
0.3 |
ns |
7. AZ: Cochise |
J1/J2 |
1.00 |
10.0 |
0.002 |
|||
8. AZ: Cochise |
J1/J2 |
1.00 |
10.0 |
0.002 |
0.09 |
0.08 |
ns |
9. AZ: Cochise |
J1/J2 |
1.00 |
10.0 |
0.002 |
0.12 |
1.4 |
ns |
10. NM: Hidalgo |
J1/J2 |
1.00 |
10.0 |
0.002 |
|||
11. NM: Grant |
J1/J2 |
1.00 |
10.0 |
0.002 |
0.08 |
0.01 |
ns |
12. NM: Chaves |
B |
0.18 |
0.26 |
ns |
|||
|
|||||||
13. TX: Oldham |
B |
0.00 |
- |
- |
|||
|
|||||||
14. TX: Potter |
B |
0.00 |
- |
- |
0.55 |
0.32 |
ns |
|
|||||||
15. TX: Carson |
B |
0.02 |
- |
- |
|||
16. OK: Cleveland |
B |
0.07 |
- |
- |
0.60 |
0.72 |
ns |
|
|||||||
17. TX: Lubbock |
B |
0.08 |
- |
- |
|||
|
|||||||
18. TX: Gaines |
B |
0.00 |
- |
- |
|||
19. TX: Scurry |
B |
0.12 |
0.01 |
ns |
|||
20. TX: Travis |
B |
0.18 |
0.1 |
ns |
0.48 |
0.51 |
ns |
P. rugosus |
|||||||
21. NV: Mineral |
R |
0.00 |
- |
- |
0.00 |
- |
- |
22. NV: Esmeralda |
R |
0.00 |
- |
- |
|||
23. NV: Nye |
R |
0.00 |
- |
- |
|||
24. NV: Clark |
R |
0.00 |
- |
- |
|||
25. AZ: La Paz |
R |
0.05 |
- |
- |
0.63 |
0.6 |
ns |
26. AZ: Yavapai |
R |
0.00 |
- |
- |
|||
27. AZ: Maricopa |
R |
0.08 |
- |
- |
|||
28. AZ: Pinal |
R |
0.03 |
- |
- |
0.45 |
0.03 |
ns |
29. AZ: Maricopa |
R |
0.00 |
- |
- |
|||
30. AZ: Pinal |
R |
0.08 |
0.01 |
ns |
|||
31. AZ: Navajo |
R |
0.30 |
- |
- |
|||
32. AZ: Graham |
R |
0.15 |
- |
- |
0.20 |
0.09 |
ns |
33. AZ: Cochise |
R |
0.15 |
- |
- |
|||
34. AZ: Cochise |
H1/H2 |
1.00 |
10.0 |
0.002 |
|||
35. AZ: Cochise |
R |
0.40 |
0.14 |
ns |
|||
36. NM: Hidalgo |
H1/H2 |
1.00 |
20.0 |
0.00007 |
0.78 |
8.6 |
0.003 |
37. NM: Grant |
R (2) |
0.67 |
- |
- |
|||
37. NM: Grant |
H1/H2 (12) |
1.00 |
12.0 |
0.0005 |
0.93 |
7.8 |
0.005 |
38. NM: Socorro |
R |
0.45 |
0.15 |
ns |
|||
39. NM: Valencia |
R |
0.23 |
0.3 |
ns |
|||
40: NM: Torrance |
R |
0.38 |
0.41 |
ns |
|||
41. NM: Guadalupe |
R (6) |
0.42 |
- |
- |
|||
41. NM: Guadalupe |
H1/H2 (13) |
1.00 |
13.0 |
0.0003 |
|||
42. TX: Potter |
H1/H2 |
1.00 |
10.0 |
0.002 |
1.00 |
10.0 |
0.001 |
43. NM: Dona Ana |
R (5) |
0.37 |
- |
- |
|||
43. NM: Dona Ana |
H1/H2 (12) |
1.00 |
12.0 |
0.0005 |
|||
44. NM: Eddy |
H1/H2 |
1.00 |
10.0 |
0.002 |
|||
45. TX: Gaines |
H1/H2 |
1.00 |
10.0 |
0.002 |
|||
46. TX: Dawson |
H1/H2 |
0.98 |
10.0 |
0.002 |
0.98 |
9.6 |
0.002 |