Ecological Archives E086-111-A1

Tia-Lynn Ashman, Megan Bradburn, Denise H. Cole, Bernard H. Blaney, and Robert A. Raguso. 2005. The scent of a male: the role of floral volatiles in pollination of a gender dimorphic plant. Ecology 86:2099–2105.

Appendix A (Table A1). A summary of floral volatiles detected by static head space analysis (SPME) from Fragaria virginiana. See Notes at end of table for details.

	Retention Time (minutes)		Hermaphrodites	Female	Buds, Calyx	Petals	Anthers
Scent compounds	ECwax	EC5	N = 7	6	13	4	6
Monoterpenoids
-pinene^†	2.26	8.94	19.71 ą 10.13	5.44 ą 2.96	+
-pinene^†	3.42	9.26	5.57 ą 2.66	1.63 ą 0.85	+
-myrcene^†	4.65	9.83	2.80 ą 0.98	2.56 ą 1.56	+
dl-limonene^†	5.35	10.62	5.75 ą 0.67	12.37 ą 5.30	+	+
unidentified monoterpene	5.85	ND	2.03 ą 0.30	3.85 ą 0.69	+
Z- -ocimene^†	6.10	10.65	4.88 ą 1.11	2.71 ą 1.37	+
E- -ocimene^†	6.42	10.85	11.31 ą 4.82	4.15 ą 1.48	+
E-furanoid linalool oxide^†	9.47	11.39	T	T	+
Z-furanoid linalool oxide^†	9.87	11.65	T	T	+
linalool^†	10.95	11.76	T	T	+
myrtenal	12.15	12.25	T	T	+
-terpineol^†	12.75	13.40	T	T
-citronellol^†	13.68	13.80	T	T	+
nerol^†	13.95	14.05	T	T	+
myrtenol	14.00	13.53	T	T
geraniol^†	14.43	14.60	T	T	+
Sesquiterpenoids
E-4,8-dimethyl-nona-1,3,7-triene^†	7.46	12.03	2.72 ą 0.56	9.45 ą 4.28	+
-cubebene^†	10.06	15.91	0.14 ą 0.14	0.33 ą 0.28	+
-ylangene^‡	10.19	16.32	11.83 ą 4.14	17.33 ą 8.91	+
-caryophyllene^†	11.63	17.06	1.10 ą 0.66	2.60 ą 1.24	+
-elemene^†	11.73	16.52	0.77 ą 0.59	1.21 ą 0.99	+
E- -farnesene^‡	12.46	17.04	T	T	+
germacrene-D^‡	13.14	17.89	6.47 ą 3.97	14.64 ą 4.97	+		+
bicyclogermacrene	13.43	18.13	0.61 ą 0.36	1.86 ą 1.76	+
E,E- -farnesene^†	13.53	17.82	T	T	+
Aromatics
anisole^†	8.33	8.52	3.72 ą 2.22	1.41 ą 0.46
benzaldehyde^†	10.57	9.40	8.94 ą 1.71	8.93 ą 4.84	+	+
methyl salicylate^†	13.91	13.48	1.68 ą 0.71	2.11 ą 1.11
benzyl alcohol^†	14.73	10.70	5.88 ą 1.79	5.63 ą 0.42
2-phenylethanol^†	15.12	12.10	2.88 ą 0.90	T			+
p-anisaldehyde^†	16.53	14.38	T	T	+
Fatty Acid Derivatives
Z-3-hexenyl acetate^†	7.55	10.07	W	W	+
Z-3-hexen-1-ol^†	8.53	7.24	W	W	+	+
hexyl isobutyrate^†	9.85	13.90	W	W	+
E-2-hexenyl butyrate^†	10.04	13.20	W	W	+
Z-3-hexenyl compound	10.22	xxxx	W	W	+
Z-3-hexenyl compound	12.57	xxxx	W	W	+
Z-3-hexenyl benzoate^†	17.45	18.70	W	W

Notes: Scent was collected from fresh intact hermaphrodite or female flowers or hermaphrodite buds (or calyces), or excised hermaphrodite petals or anthers. Values are percent of total bend (mean ą SE). Notations are as follows: T: Only trace amounts (less than 0.01% of total) were detected or the amounts were at the threshold of detection and too variable between samples. W: These are wound-related artifacts which were identified but excluded from consideration. ND: not detected by SPME.

† Compound identity was verified by co-retention with known standards on 2 GC columns.

‡ Compounds are consistent with published accounts of relative retention time, but no standard was available.

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