Ecological Archives A025-074-A1

Yuting Liang, Xu Zhang, Jizhong Zhou, and Guanghe Li. 2015. Long-term oil contamination increases deterministic assembly processes in soil microbes. Ecological Applications 25:12351243. http://dx.doi.org/10.1890/14-1672.1

Appendix A. Tables and figures showing dissimilarity among all the five sites and between pairwise sites by NPMANOVA (Table A1, A2), and decrease of microbial functional genes richness (Fig. A1) and diversity (Fig. A2A6) along with long-term oil contamination.

Table A1. Dissimilarity test among all the five sites by NPMANOVA.

 

r value

P

Uncontaminated soils

2.880

< 0.0001

Contaminated soils

1.601

0.0002

 

Table A2. Dissimilarity test between pairwise sites by NPMANOVA.

Uncontaminated soils

 

Contaminated soils

r value\P

BS

DQ

JH

SL

TLM

 

BS

DQ

JH

SL

TLM

BS

 

0.0002

0.0007

0.0001

0.0002

 

 

0.1427

0.0106

0.1611

0.0305

DQ

2.619

 

0.0002

0.0002

0.0003

 

1.191

 

0.0176

0.0726

0.0004

JH

2.980

2.553

 

0.0019

0.0001

 

1.817

1.617

 

0.0266

0.0001

SL

2.658

2.959

2.260

 

0.0098

 

1.223

1.418

1.723

 

0.1560

TLM

4.235

3.938

2.979

2.051

 

 

1.612

1.937

2.320

1.228

 

 

FigA1

Fig. A1. Microbial functional genes (richness) decrease significantly with long-term oil contamination.


 

FigA2

Fig. A2. Decrease in microbial diversity (Shannon index) of all functional genes with oil concentrations across the five sites.


 

FigA3

Fig. A3. Decrease in microbial diversity (Shannon index) of functional genes in nitrogen cycling with oil concentrations across the five sites. nifH is active in nitrogen fixation, and nirS/K, nos in denitrification.


 

FigA4

Fig. A4. Decrease in microbial diversity (Shannon index) of functional genes in carbon fixation with oil concentrations across the five sites. Rubisco (ribulose-l, 5-bisphosphate carboxylase/oxygenase) is expressed in the Calvin cycle, CODH (carbon monoxide dehydrogenase) in the reductive acetyl-CoA pathway, and pcc (propionyl-CoA/acetyl-CoA carboxylase) in the 3-hydroxypropionate/malyl-CoA cycle.


 

FigA5

Fig. A5. Decrease in microbial diversity (Shannon index) of functional genes in carbon degradation (starch, cellulose, chitin and lignin) with oil concentrations across the five sites.


 

FigA6

Fig. A6. Decrease in microbial diversity (Shannon index) of functional genes in phosphorus utilization with oil concentrations across the five sites. ppk and ppx encode polyphosphate kinase and exopolyphosphatase respectively.


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