Ecological Archives M077-005-A4

Paul Kardol, Nelleke J. Cornips, Monique M. L. van Kempen, J. M. Bakx-Schotman, and Wim H. van der Putten. 2007. Microbe-mediated plant–soil feedback causes historical contingency effects in plant community assembly. Ecological Monographs 77:147–162.

Appendix D. Temperature touch-down cycling programs for bacterial and fungal PCR.

Bacterial PCR

 

Fungal PCR

1

94 °C

2 min

 
 

1

94 °C

4 min

 

2

92 °C

30 s

 
 

2

92 °C

30 s

 

3

65 °C

1 min

 
 

3

55 °C

1min

 

4

68 °C

2 min

 
 

4

68 °C

2 min

 

5

go to 2

1 time

 
 

5

go to 2

1 time

 

6

92 °C

30 s

 
 

6

92 °C

30 s

 

7

63 °C

1min

 
 

7

53 °C

1min

 

8

68 °C

2 min

 
 

8

68 °C

2 min

 

9

go to 6

1 time

 
 

9

go to 6

1 time

 

10

92 °C

30 s

 
 

10

92 °C

30 s

 

11

61 °C

1min

 
 

11

51 °C

1min

 

12

68 °C

2 min

 
 

12

68 °C

2 min

 

13

go to 10

1 time

 
 

13

go to 10

1 time

 

14

92 °C

30 s

 
 

14

92 °C

30 s

 

15

59 °C

1min

 
 

15

49 °C

1min

 

16

68 °C

2 min

 
 

16

68 °C

2 min

 

17

go to 14

1 time

 
 

17

go to 14

1 time

 

18

92 °C

30 s

 
 

18

92 °C

30 s

 

19

57 °C

1min

 
 

19

47 °C

1min

 

20

68 °C

2 min

 
 

20

68 °C

45 s

1 s/cycle

21

go to 18

1 time

 
 

21

go to 18

31 times

 

22

92 °C

30 s

 
 

22

68 °C

10 min

 

23

55 °C

1min

 
   
 
 
 

24

68 °C

45 s

1 s/cycle

   
 
 
 

25

go to 22

24 times

 
   
 
 
 

26

68 °C

10 min

 
   
 
 
 


[Back to M077-005]