Ecological Archives E093-145-A4

Jessica A. Bryant, Frank J. Stewart, John M. Eppley, and Edward F. DeLong. 2012. Microbial community phylogenetic and trait diversity declines with depth in a marine oxygen minimum zone. Ecology 93:1659–1673. http://dx.doi.org/10.1890/11-1204.1

Appendix D. Additional Eastern Tropical South Pacific (ETSP) diversity figures.

Fig. D1. Broad taxonomic distribution of SSU genes at each sampling depth in (A) 2008, (B) 2009, and (C) 2010.


Fig. D2. Taxonomic richness across depth at the ETSP: orange squares represent 2008 samples, red circles represent 2009 samples and blue triangles represent 2010 data. Rarefaction analysis was used to account for differences in sequencing depth between samples by normalizing all samples in the figure to the smallest ETSP sample in the study (ETSP 2008 800 m sample; see Methods section).


Fig. D3. (A) Taxonomic richness and (B) phylogenetic diversity of non-eukaryotic taxa across depth at the ETSP: orange squares represent 2008 samples, red circles represent 2009 samples and blue triangles represent 2010 data. Rarefaction analysis was used to account for differences in sequencing depth between samples by normalizing all samples in the figure to the smallest ETSP sample in the study (ETSP 2008 800 m sample; see Methods section).


Fig. D4. Functional richness based on clustering protein sequences (FRPC) at the ETSP in the (A) 2008 depth series and in the (B) 2009 and 2010 depth series. Orange squares represent 2008 samples, red circles represent 2009 samples and blue triangles represent 2010 data. Rarefaction analysis was used to account for differences in sequencing depth between samples by normalizing all samples in the figure to the smallest ETSP sample in the study (ETSP 2008 800 m sample; see Methods section).


Fig. D5. Abundance based MPD (aMPD) of (A) all taxa in each ETSP sample and (B) non-eukaryotic taxa in each ETSP sample across depth. Colored shapes (by sample year, as in Fig. D2) represent abundance-weighted mean phylogenetic distance (MPD) between all pairs of taxa identified in each sample. Black bars outline 95% confidence interval expected from randomly generated communities. Blue shading outlines regions of the water column with oxygen concentrations below 5 μM. Rarefaction analysis was used to account for differences in sequencing depth between samples within the same depth series (year; see Methods section).


Fig. D6. Phylogenetic dispersion (MPD) of non-eukaryotic taxa across depths in the ETSP. Colored shapes (by sample year, as in Fig. D2) represent mean phylogenetic distance (MPD) between all pairs of taxa identified in each sample. Black bars outline 95% confidence interval expected from randomly generated communities. Blue shading outlines regions of the water column with oxygen concentrations below 5 μM. Rarefaction analysis was used to account for differences in sequencing depth between samples within the same depth series (year; see Methods section).


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